Method for the determination of selected organochlorine pesticides and polychlorinated biphenyls in human serum based on a gel permeation chromatographic clean-up

A gel permeation chromatographic (GPC) clean-up based method was developed for determination of selected organochlorine pesticides and polychlorinated biphenyls in human serum. The method permits automation of the sample extract clean-up stage and is designed to work with small amounts of sample. Different working variables were studied in its development, including injection volume, flow rate, and fat amount as the most representative coextract. The method provides solvent and time savings. Initial extraction was performed using 96-well solid-phase disk extraction plates, and quantification was performed by gas chromatography with electron capture detection and mass spectrometry. Recoveries of PCB congeners 28, 52, 101, 118, 138, 153, and 180 and organochlorine pesticides HCB, β-HCH, γ-HCH, heptachlor epoxide, p,p′-DDE, and p,p′-DDT at two spiking levels ranged from 55% to 115% with relative standard deviations ranging from 2.0% to 14.6%. Limits of quantification ranged from 0.06 to 0.16 ng mL⁻¹ for PCBs and from 0.12 to 0.36 ng mL⁻¹ for organochlorine pesticides. Finally, the method was applied to the analysis of 38 serum samples and the results were compared with those of another procedure validated at the laboratory.     

Charcoal burning as a source of polyaromatic hydrocarbons in waterpipe smoking

Polyaromatic hydrocarbons (PAH) content from seven commercial waterpipe charcoals were determined during the smoking process to estimate how much PAHs would not be trapped by the water trap and could reach the lungs of the smokers. Naphthalene, 2-methylnaphthalene, acenaphthylene, acenaphthene, phenanthrene and fluoranthene were the most abundant PAH compounds produced during smoking. Naphthalene was the highest in all of the smoke contents and levels of 5 to 405 μg/15 minutes could be inhaled by the smoker. The amounts of PAHs produced during the smoking events in absence of tobacco varied greatly among different brands of charcoal. The amount and composition of the emitted PAH were not related to the amount and composition of the original chemicals in the charcoal prior to burning. Our findings suggest that public health agencies should regulate smoked charcoal products alongside tobacco.     

Road safe seniors: Screening for age-related driving disorders in inpatient and outpatient settings

Introduction

Older drivers are increasing in number and they often have health conditions that place them at high risk for motor-vehicle crashes (MVC). Screening is underutilized, and is rarely done in hospital settings.

Methods

A convenience sample of 755 older adults completed age related driving disorders screening at University of California, San Diego inpatient and outpatient health centers. Screening included three strength/frailty tests, two vision tests (acuity and fields), and two cognitive tests, based on AMA recommendations. The average age of participants was 72.5; 55.5% were male and 94% English-speaking; 17.8% of older adults failed at least one aspect of screening.

Results

In multivariate analysis, significant associations of failed status were age, male sex, selfrestrictions of driving, and inpatient screening locations. The screening identified one in six adults to be 'high-risk' for age related driving disorders. Screening was effective and feasible in both inpatient and outpatient settings.

Impact on industry

As the driving population ages, industry, government and health car providers need to plan for the management of driving impairments in older adults.     

Understanding slums: analysis of the metabolic pattern of the Vidigal favela in Rio de Janeiro,Brazil

This paper illustrates an innovative approach to characterize the metabolic pattern of informal urban settlements or slums with the aim to better understand the factors that affect the material standard of living of slum residents, the dynamics of slum development and the interaction of the slum with its wider socioeconomic context. The proposed system of accounting, multi-scale integrated analysis of societal and ecosystem metabolism (MuSIASEM), integrates socioeconomic and spatial data and studies energy and monetary flows in relation to the pattern of human activities and land uses. The theoretical basis of the approach is illustrated with data from Vidigal favela in Rio de Janeiro, Brazil. In particular, we show how to construct taxonomies of accounting categories to characterize: (1) the set of activities carried out by the slum dwellers, to which to link assessments of flow rates per hour; (2) the set of land uses or spatial elements making up the slum, to which to link assessments of flow densities per hectare. The analysis of the interaction of Vidigal with its wider socioeconomic context focuses on monetary flows and transport (job commuting).     

Nanoweapon: control of mosquito breeding using carbon-dot-silver nanohybrid as a biolarvicide

Health issues induced by mosquito illnesses highlight the need for effective control. Here, we developed an efficient carbon-dot-silver nanohybrid to control Anopheles stephensi and Culex quinquefasciatus mosquito species. The nanohybrid was synthesized using a thermal method without addition of any toxic-reducing agent. Monohybrids are found within the ranges of 2–6 nm for carbon-dot and 10–35 nm for silver nanoparticles with uniform distribution. The uniformly dispersed nanohybrid solutions show excellent larvicidal activity within the concentration range of 0.5–1.0 ppm. Morphological studies evidence the presence of strong bonds between nanohybrid and sulphur- or phosphorus-containing compounds such as proteins and DNA present in the larval body. This explains tissue damage at very low concentrations of nanohybrid. Therefore, this nanoweapon has high potential for field applications.     

SIMULATION AS AN AID TO DECISION MAKING IN A WATER UTILITY1

ABSTRACT. Middle and high management levels within water utilities would find their decision making activities greatly enhanced if provided with a set of techniques having the following characteristics: (1) the ability to procure information and knowledge about real-life systems, (2) the ability to promote analysis of the real life system and (3) the capacity to gauge the impact of decisions. A model/simulation is presented, having the capability to mime operational aspects of water supply systems. The simulation produces time series of what are considered relevant operational variables. These series are amenable to analysis of both static and dynamic effects of alternative policies, changing environmental conditions and varying parametric specifications. Because of its modular structure and the ad hoc programming language utilized, it offers great flexibility. The model/simulation allows extensions, deletions and modifications without consequent reformulation or extensive reprogramming. It performs a number of statistical tests useful for its own verification and validation. Also available are a suggested methodology and procedures for model use, possible difficulties with data gathering and operation, plus an idea of what cannot be done with the currently extant model version.     

Tragedy of the Temporal Commons: Soil-Bound Lead and the Anachronicity of Risk

In 2002, a team of researchers from the University of California, Irvine collaborated with the community of Pacoima, California around a co-ordinated effort to analyze soil around the neighborhood for lead. The team found both total and bioavailable lead to be markedly higher in areas close to major highways, almost 20 years after leaded gasoline had been completely phased out. Multi-regression and cluster analyses reveal the association of elevated levels of total and bioavailable lead with proximity to major highways that cut through Pacoima. Moreover, bioavailability ratios are higher next to highways than elsewhere. What this reveals is an unexpected persistence of lead deposited by vehicular emissions over a long period of time, a potentially intractable policy issue. The long residence time of soil lead represents an enduring public health problem, especially considering the numbers of those potentially exposed over time. It is unclear how expedient or realistic the conversion of land use around major highways might be, or how this new information might be integrated into ongoing movements for change. However, some policy actions can, even now, be considered—e.g. a closer policy focus on the bioavailable, not just total, fraction of soil lead. We also reflect upon how these traces in the soil give us a more profound sense of the cumulative burden that some communities have to bear due to a history of neglect.     

Serum levels of organochlorine pesticides in healthy adults from five regions of Spain

The aim of this study was to measure of serum levels of p,p′-dichlorodiphenyl trichloroethane (p,p′-DDT), p,p′-dichlorodiphenyl dichlorethylene (p,p′-DDE), β-hexachlorocyclohexane (β-HCH), and hexachlorobenzene (HCB) in healthy adults in Spain. Furthermore, we also analyzed these levels according to dietary, other lifestyle factors and anthropometric characteristics. We measured the concentrations of such organochlorine pesticides (OCPs) in serum samples collected during 1992–1996 from 953 subjects aged 35–64 years, they were residents of five Spanish regions, they were randomly selected from the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. OCPs were determined by means of gas chromatography with electron-capture detection (GC-ECD). The most frequent compound found in serum was p,p′-DDE, present in 98% of the samples, followed by HCB and β-HCH, found in 89% and 77% of samples, respectively, while p,p′-DDT could be measured only in 26% of subjects. The geometric means of serum concentrations (ng/g lipid) were 822 for p,p′-DDE, 167 for β-HCH, and 379 for HCB. The concentrations of all OCPs were positively associated with age and body mass index, and decreased along the period of blood collection. No association was found between OCPs levels and dietary factors. The concentrations of p,p′-DDE and β-HCB were higher in Murcia, one of southern regions, most likely associated with intensive past use of pesticides related to agricultural practices, while higher levels of HCB were found in Navarra, located in the north, maybe due to industrial use rather than agricultural application.     

Occurrence of Serious Injury in Real-World Side Impacts of Vehicles with Good Side-Impact Protection Ratings

Objective: The Insurance Institute for Highway Safety (IIHS) introduced its side impact consumer information test program in 2003. Since that time, side airbags and structural improvements have been implemented across the fleet and the proportion of good ratings has increased to 93% of 2012–2014 model year vehicles. Research has shown that drivers of good-rated vehicles are 70% less likely to die in a left-side crash than drivers of poor-rated vehicles. Despite these improvements, side impact fatalities accounted for about one quarter of passenger vehicle occupant fatalities in 2012. This study is a detailed analysis of real-world cases with serious injury resulting from side crashes of vehicles with good ratings in the IIHS side impact test.

Methods: NASS-CDS and Crash Injury Research and Engineering Network (CIREN) were queried for occupants of good-rated vehicles who sustained an Abbreviated Injury Scale (AIS) ≥ 3 injury in a side-impact crash. The resulting 110 cases were categorized by impact configuration and other factors that contributed to injury. Patterns of impact configuration, restraint performance, and occupant injury were identified and discussed in the context of potential upgrades to the current IIHS side impact test.

Results: Three quarters of the injured occupants were involved in near-side impacts. For these occupants, the most common factors contributing to injury were crash severities greater than the IIHS test, inadequate side-airbag performance, and lack of side-airbag coverage for the injured body region. In the cases where an airbag was present but did not prevent the injury, occupants were often exposed to loading centered farther forward on the vehicle than in the IIHS test. Around 40% of the far-side occupants were injured from contact with the struck-side interior structure, and almost all of these cases were more severe than the IIHS test. The remaining far-side occupants were mostly elderly and sustained injury from the center console, instrument panel, or seat belt. In addition, many far-side occupants were likely out of position due to events preceding the side impact and/or being unbelted.

Conclusion: Individual changes to the IIHS side impact test have the potential to reduce the number of serious injuries in real-world crashes. These include impacting the vehicle farther forward (relevant to 28% of all cases studied), greater test severity (17%), the inclusion of far-side occupants (9%), and more restrictive injury criteria (9%). Combinations of these changes could be more effective.     

Correction to: Ecological restoration as a strategy for mitigating and adapting to climate change: lessons and challenges from Brazil

Mitigation and Adaptation Strategies for Global Change - The original version of the article unfortunately contained an error.